The results showed that the growth of root tips in liquid medium was better than that in agar - solidified medium and solid - liquid two phase medium , for that increasing agar concentrations in medium will reduce the root growth ; darkness was beneficial for fraxinus mandshurica root tip culture in vitro ; as the carbon sources , sucrose was better than glucose and maltose as the carbon sources , and 3 % sucrose was best for the root tip growth of fraxinus mandshurica 結果表明,不添加瓊脂的液體培養(yǎng)基對根尖的生長較添加瓊脂的固體培養(yǎng)基和固液培養(yǎng)基好,原因是增加瓊脂的量會抑制根的生長;暗培養(yǎng)比光培養(yǎng)更有利于水曲柳離體根尖的培養(yǎng);蔗糖作為碳源的效果較果糖和麥芽糖好,其中以3 %的蔗糖對離體根尖的生長效果最好。